|
KMID : 0359319990390040751
|
|
Korean Journal of Veterinary Research
1999 Volume.39 No. 4 p.751 ~ p.759
|
|
|
Viral characteristics of plaque variants of porcine reproductive and respiratory syndrome virus
|
|
Park, Bong Kyun
Joo, Han Soo/Molitor, Thomas W .
|
|
|
Abstract
|
|
|
|
Plaque characteristics of porcine reproductive and respiratory syndiome (PRRS) virus isolates were examined using MARC-145 line cells. The plaque morphology of PRRS virus isolates was variable in size and heterogenic in population. Upon serial passages of the PRRS virus isolates on MARC-145 tells, heterogeneity was maintained but numbers of the large plaque size virus were increased with certain isolates. A PRRS virus isolate with variable plaque sizes was subcloned into 2 populations : small plaque (H_S) and large plaque (H_L) viruses. Growth kinetics of the subclones were then determined in MARC-145 cells, and production of the structural polypeptides was analyzed by SDS-PAGE. In a comparison of the growth kinetics, the H_S virus showed higher infectivity titers during the first 48 hours but slower to reach the peak titier than H_L virus did. In a nucleotide sequence comparison, differences of 4 nucleotides in open reading frames 5-6 gene were found between H_S and H_L viruses. Both the H_S and H_L clones produced 5 polypeptide bands with molecular weights of 15, 19, 26, 36 and 42 kD. The 5 bands were detected at 48 hours postinoculation (PI) with antisera to H_L and another large plaque virus (W_L) and at 72 hours PI with H_S virus antiserum. The present results demonstrate differences of biologic and molecular characteristics between the two PRRS virus plaque clones.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|